The heart-stopping footage shows the Russian military jet zooming past the US Navy aircraft while flying over the Black Sea on Monday.
US officials have slammed the move, claiming it was "unsafe" and "irresponsible" as it threatened the lives of all pilots and crew on board.
Pentagon spokesman Eric Pahon said the US Airborne Turbulence and Vibration hit the jet during the 25-minute ordeal.
He added there is no communication between the two jets.
The US Navy therefore claimed the aircraft had been flying in international law and did not provoke the Russian reaction.
The US Navy said in a statement: "This interaction is very dangerous," the US Navy said in a statement.
"The intercepting SU-27 made an additional pass, closing with the EP-3 and applying its afterburner while conducting a banking turn-away."
The Russian Embassy posted a letter on Twitter denying any fault.
"The SU-27 jet's crew reported identifying the #US EP-3 Aries spy plane and listened to it," the tweet said.
It is not the first high-profile encounter between two nations in the skies.
In January, the Russian jet intercepted a US aircraft and came within just five feet over the Black Sea.